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TRC-00802 | Total Aflatoxin ELISA Kit | 96 Well

Product Overview

A competitive ELISA kit for the quantitative measurement of Total Aflatoxin in Wine, Soy, sauce, vinegar, Grain, Peanut, Formula feed, Edible oil.

This Total Aflatoxin ELISA Kit is based on the Competitive ELISA method. It can detect Aflatoxin (AF) in samples, such as grain, peanut, formula feed, etc. The microplate provided in this kit has been pre-coated with coupled antigen. During the reaction, AF in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-AF antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of AF. The concentration of AF in the samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Information

Assay FormatCompetitive ELISA
Species ReactivityUniversal
Sample TypeWine, Soy, sauce, vinegar, Grain, Peanut, Formula feed, Edible oil
Detection MethodAbsorbance (450 nm)
Detection Wavelength450 nm
Kit Size96 Well

Performance Data

Sensitivity0.02 ppb (ng/ml), Sample recovery rate: Grain/Formula feed- 85%±15%, Peanut- 82%±15%, Edible oil- 85%±15%, Sauce/Wheat/Barley feed- 83%±15%, Beer- 84%±15%, Wine/Soy sauce/Vinegar- 87%±15%
Detection LimitGrain- 0.1 ppb

Applications

This ELISA kit is used for quantitative measurement of Total Aflatoxin in Wine, Soy, sauce, vinegar, Grain, Peanut, Formula feed, Edible oil.

Features & Benefits

  • Designed for quantitative measurement of Total Aflatoxin.
  • Assay format: Competitive ELISA.
  • Sample type: Wine, Soy, sauce, vinegar, Grain, Peanut, Formula feed, Edible oil.
  • Detection method: Absorbance (450 nm).
  • Sensitivity: 0.02 ppb (ng/ml), Sample recovery rate: Grain/Formula feed- 85%±15%, Peanut- 82%±15%, Edible oil- 85%±15%, Sauce/Wheat/Barley feed- 83%±15%, Beer- 84%±15%, Wine/Soy sauce/Vinegar- 87%±15%.
  • Kit size: 96 Well.

Storage & Shipping

Storage+4°C
ShippingGel Pack
Shelf Life12 Months

Product Details

Catalog NumberTRC-00802
Analyte / TargetTotal Aflatoxin
Alternate Name3110