TRC-00782 | Thyroxine (T4) (Mouse/Rat) ELISA Kit | 96 Well
Product Overview
Sensitive, Colorimetric Assay
T4 is a useful marker for the diagnosis of hypothyroidism and hyperthyroidism. The level of T4 is decreased in hypothyroid patients and is increased in hyperthyroid patients. 96Trace’s mouse/rat thyroxine (T4) kit is a solid phase competitive ELISA Kit. The samples, and T4 enzyme conjugate are added to the wells coated with anti-T4 polyclonal antibody. T4 in the sample competes with a T4 enzyme (HRP) conjugate for binding sites. Unbound T4 and T4 enzyme conjugate are washed off by wash buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of T4 in the samples. A standard curve is prepared relating color intensity to the concentration of the T4.
Assay Information
| Assay Format | Competitive ELISA |
| Species Reactivity | Mouse/rat |
| Sample Type | Serum or plasma |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Sensitivity | 0.467 μg/dl |
Applications
This ELISA kit is used for quantitative protein detection, establishing normal range.
Features & Benefits
- Easy, convenient and time-saving method to assay for mouse/rat thyroxine.
- Sensitivity: 0.467 μg/dl.
Storage & Shipping
| Storage | 2-8°C |
| Shipping | Gel Pack |
| Shelf Life | 24 Months |
Product Details
| 96Trace CAT | K7421-100 |
| Supplier CAT | TRC-00782 |
| Analyte / Target | Thyroxine (T4) |
| Category | Cytokine & Growth Factor ELISA Kits |