TRC-00745 | Sulfametoxydiazine ELISA Kit | 96 Well
Product Overview
A competitive ELISA kit for the quantitative measurement of Sulfametoxydiazine in Honey, Milk, Serum, Tissue lysate, Urine.
Sulfametoxydiazine (INN) or sulfamethoxydiazine (USAN: sulfameter) is a long-acting sulfonamide antibacterial. It is used as a leprostatic agent and in the treatment of urinary tract infections. 96Trace Sulfametoxydiazine ELISA Kit is based on the Competitive ELISA principle. It can detect Sulfametoxydiazine in tissue, serum, honey, milk, urine samples. The micro-plate provided in this kit has been pre-coated with Sulfametoxydiazine. During the reaction, Sulfametoxydiazine in the samples or standard competes with Sulfametoxydiazine coated on the plate for binding to the anti-Sulfametoxydiazine antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of Sulfametoxydiazine. The concentration of Sulfametoxydiazine in the samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Information
| Assay Format | Competitive ELISA |
| Species Reactivity | Universal |
| Sample Type | Honey, Milk, Serum, Tissue lysate, Urine |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Sensitivity | 0.05 ppb (ng/mL), Sample recovery rate: Tissue/honey - 95%±25%, Urine/milk/serum - 85%±25%, Detection limit: Tissue (high detection limit) - 0.5 ppb, Tissue (low detection limit) - 0.25 ppb, Serum/urine - 0.2 ppb, Honey - 0.05 ppb, Milk - 1 ppb, Cross-reactivity: Sulfadiazine (SD or SDZ) - 40%, Sulfamerazine (SM1) - 25%, Sulfadoxine (SDM) - 35% |
| Detection Limit | Tissue (high detection limit) - 0.5 ppb, Tissue (low detection limit) - 0.25 ppb, Serum/urine - 0.2 ppb, Honey - 0.05 ppb, Milk - 1 ppb, Cross-reactivity: Sulfadiazine (SD or SDZ) - 40%, Sulfamerazine (SM1) - 25%, Sulfadoxine (SDM) - 35% |
Applications
This ELISA kit is used for quantitative measurement of Sulfametoxydiazine in Honey, Milk, Serum, Tissue lysate, Urine.
Features & Benefits
- Designed for quantitative measurement of Sulfametoxydiazine.
- Assay format: Competitive ELISA.
- Sample type: Honey, Milk, Serum, Tissue lysate, Urine.
- Detection method: Absorbance (450 nm).
- Sensitivity: 0.05 ppb (ng/mL), Sample recovery rate: Tissue/honey - 95%±25%, Urine/milk/serum - 85%±25%, Detection limit: Tissue (high detection limit) - 0.5 ppb, Tissue (low detection limit) - 0.25 ppb, Serum/urine - 0.2 ppb, Honey - 0.05 ppb, Milk - 1 ppb, Cross-reactivity: Sulfadiazine (SD or SDZ) - 40%, Sulfamerazine (SM1) - 25%, Sulfadoxine (SDM) - 35%
- Kit size: 96 Well.
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 12 Months |
Product Details
| Catalog Number | TRC-00745 |
| Analyte / Target | Sulfametoxydiazine |
| Alternate Name | 3110 |