TRC-00743 | Sulfamethazine ELISA Kit | 96 Well
Product Overview
A competitive ELISA kit for the quantitative measurement of Sulfamethazine in Tissue, Serum, Honey, Milk, Egg, Urine.
Sulfamethazine is a sulfonamide consisting of pyrimidine with methyl substituents at the 4- and 6-positions and a 4-aminobenzenesulfonamido group at the 2-position. It has a role as an anti-infective agent, a carcinogenic agent, a ligand, an antibacterial drug, an antimicrobial agent. Sulfamethazine ELISA Kit is based on the Competitive ELISA principle. The micro-plate provided in this kit has been pre-coated with Sulfamethazine. During the reaction, Sulfamethazine in the samples or standard competes with Sulfamethazine coated on the plate for binding to the anti-Sulfamethazine antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of Sulfamethazine. The concentration of Sulfamethazine in the samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Information
| Assay Format | Competitive ELISA |
| Species Reactivity | Universal |
| Sample Type | Tissue, Serum, Honey, Milk, Egg, Urine |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Sensitivity | 0.5 ppb (ng/mL), Sample recovery rate: Tissue, Honey- 95%±25%, Urine, Milk, Serum- 85%±25%., Detection limit: Tissue (high detection limit method) - 0.5 ppb, Tissue (low detection limit method) - 2.5 ppb, Serum, Urine - 2 ppb, Honey- 0.5 ppb, Milk -10 ppb |
| Detection Limit | Tissue (high detection limit method) - 0.5 ppb, Tissue (low detection limit method) - 2.5 ppb, Serum, Urine - 2 ppb, Honey- 0.5 ppb, Milk -10 ppb |
Applications
This ELISA kit is used for quantitative measurement of Sulfamethazine in Tissue, Serum, Honey, Milk, Egg, Urine.
Features & Benefits
- Designed for quantitative measurement of Sulfamethazine.
- Assay format: Competitive ELISA.
- Sample type: Tissue, Serum, Honey, Milk, Egg, Urine.
- Detection method: Absorbance (450 nm).
- Cross-reactivity: Sulfamethazine - 100%., Sensitivity: 0.5 ppb (ng/mL), Sample recovery rate: Tissue, Honey- 95%±25%, Urine, Milk, Serum- 85%±25%., Detection limit: Tissue (high detection limit method) - 0.5 ppb, Tissue (low detection limit method) - 2.5 ppb, Serum, Urine - 2 ppb, Honey- 0.5 ppb, Milk -10 ppb
- Kit size: 96 Well.
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 12 Months |
Product Details
| Catalog Number | TRC-00743 |
| Analyte / Target | Sulfamethazine |
| Alternate Name | Sulfadimidine |