TRC-00737 | sTREM-1 (Human) ELISA Kit | 96 Well
Product Overview
A sandwich ELISA kit for the quantitative measurement of sTREM-1 in Plasma; Serum; Tissue lysate; Other biological fluids.
The triggering receptor expressed on myeloid cells-1 (TREM-1) is a type I transmembrane immune receptor that belongs to the TREM family of protein receptors. It is expressed on neutrophils and mature macrophages/monocytes. TREM-1 plays a significant role in the amplification of inflammation and activation of antigen-presenting cells (APC). A soluble form of TREM-1 (sTREM-1) is found in the plasma and is thought to arise from the proteolytic cleavage of membrane-associated TREM-1 by metalloproteinases (MMPs). Increased levels of sTREM-1 are observed during infection and inflammatory diseases such as inflammatory bowel disease, rheumatoid arthritis, renal IR. Therefore, sTREM-1 is a promising serological biomarker for these diseases. 96Trace’s sTREM-1 (Human) ELISA kit is based on the Sandwich principle to quantitatively measure the concentration of sTREM-1 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is directly proportional to the concentration of sTREM-1 in the sample or standard
Assay Information
| Assay Format | Sandwich ELISA |
| Species Reactivity | Human |
| Sample Type | Plasma Serum Tissue lysate Other biological fluids |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 31.25 - 2000 pg/ml |
| Sensitivity | 18.75 pg/ml |
Applications
This ELISA kit is used for quantitative measurement of sTREM-1 in Plasma; Serum; Tissue lysate; Other biological fluids.
Features & Benefits
- Designed for quantitative measurement of sTREM-1.
- Assay format: Sandwich ELISA.
- Sample type: Plasma; Serum; Tissue lysate; Other biological fluids.
- Detection method: Absorbance (450 nm).
- Detection range: 31.25 - 2000 pg/ml
- Sensitivity: 18.75 pg/ml
- Kit size: 96 Well.
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 6 Months |
Product Details
| Catalog Number | TRC-00737 |
| Analyte / Target | sTREM-1 |
| Alternate Name | TREM1, CD354, TREM-1, CD354 Antigen |