SSA IgG (Human) ELISA Kit

Product Overview

An ELISA kit for the quantitative measurement of SSA IgG in Plasma; Serum.

Sjogren syndrome (SS)A/Ro are autoantigens that are a type of extractable nuclear antigen (ENA). SSA consists of 2 proteins: Ro60 and Ro52. Ro60 binds to uncoded RNA to form an hY-RNA complex and thereby inhibits the immune response. Ro52 is a protein formed either due to viral infection, type I interferons, or Toll-like receptors. Antibodies to SSA/Ro antigen are detected in patients suffering from autoimmune diseases such as systemic lupus erythematosus (SLE), Sjögren's syndrome (SS), systemic sclerosis, and rheumatoid arthritis (RA). 96Trace’s SSA IgG (Human) ELISA kit quantitatively measures SSA IgG antibodies in human serum and plasma samples. Samples, calibrator, and controls are added to the wells pre-coated with SSA antigen. IgG antibodies to SSA, if present in the samples, will bind to the SSA antigen. The wells are then washed with Wash Buffer, followed by incubation with the enzyme conjugate. After incubation, any unattached conjugates are washed off by Wash Buffer. The enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is directly proportional to the amount of IgG antibodies to SSA present in the sample

Assay Information

Features & Benefits

• Designed for quantitative measurement of SSA IgG.
• Sample type: Plasma; Serum.
• Detection method: Absorbance (450 nm).
• Kit size: 96 Well.

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Product Details