TRC-00731 | Spectinomycin ELISA Kit | 96 Well
Product Overview
A competitive ELISA kit for the quantitative measurement of Spectinomycin in Raw milk, Tissue (muscle).
Spectinomycin is an aminocyclitol drug produced by the soil microorganism Streptomyces spectabilis. The drug is effective against gram-positive and gram-negative bacteria and is used to treat gonorrheal infections. Spectinomycin works by binding to the 30S ribosomal subunit and thus inhibiting bacterial protein synthesis. The drug is used in animals not bred for human consumption to treat bacterial respiratory and enteric infections. 96Trace’s Spectinomycin ELISA kit is used to quantitatively measure Spectinomycin in tissue and milk samples. The kit is based on the Competitive ELISA principle. Samples and standards are added to the microwell plate that is pre-coated with an antigen and competes for binding to the anti-Spectinomycin antibody. The HRP conjugate is added to each well and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of substrate reagents. Finally, the reaction is terminated with an acidic stop solution. The color developed is inversely proportional to the concentration of Spectinomycin in the samples.
Assay Information
| Assay Format | Competitive ELISA |
| Species Reactivity | Universal |
| Sample Type | Raw milk, Tissue (muscle) |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Sensitivity | 0.2ppb, Detection Limit: 5ppb for raw milk, 10ppb for muscle, Cross reaction: Spectinomycin 100%, Streptomycin, Neomycin, Kanamycin, Gentamicin, Dihydrostreptomycin all＜0.01%, This ELISA kit is used for in vitro quantitative determination of Spectinomycin |
| Detection Limit | 5ppb for raw milk, 10ppb for muscle, Cross reaction: Spectinomycin 100%, Streptomycin, Neomycin, Kanamycin, Gentamicin, Dihydrostreptomycin all＜0.01%, This ELISA kit is used for in vitro quantitative determination of Spectinomycin |
Applications
This ELISA kit is used for quantitative measurement of Spectinomycin in Raw milk, Tissue (muscle).
Features & Benefits
- Designed for quantitative measurement of Spectinomycin.
- Assay format: Competitive ELISA.
- Sample type: Raw milk, Tissue (muscle).
- Detection method: Absorbance (450 nm).
- Sensitivity: 0.2ppb, Detection Limit: 5ppb for raw milk, 10ppb for muscle, Cross reaction: Spectinomycin 100%, Streptomycin, Neomycin, Kanamycin, Gentamicin, Dihydrostreptomycin all＜0.01%, This ELISA kit is used for in vitro quantitative determination of Spectinomycin
- Kit size: 96 Well.
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 12 Months |
Product Details
| Catalog Number | TRC-00731 |
| Analyte / Target | Spectinomycin |
| Alternate Name | Spectinomycin, SCM |