Sm/RNP IgG (Human) ELISA Kit

Product Overview

An ELISA kit for the quantitative measurement of Sm/RNP IgG in Plasma; Serum.

Smith/Ribonucleoprotein (Sm/RNP, U1RNP) are autoantigens that are a type of extractable nuclear antigen (ENA). Antibodies to Sm/RNP target small nuclear ribonucleoproteins (snRNP). snRNPs are localized in the nucleus and play an important role in the processing of pre-mRNA. Antibodies to RNP target 3 autoantigens (A, C, and 68 kD); whereas antibodies to Sm target 7 autoantigens (B/B', D1, D2, D3, E, F, G). Antibodies to Sm are detected in 20% of the patients suffering from systemic lupus erythematosus (SLE). On the other hand, antibodies to RNP are detected in 45% of the patients with SLE and also in patients suffering from Sjögren’s syndrome, scleroderma, mixed connective tissue disease, and polymyositis. 96Trace’s Sm/RNP IgG (Human) ELISA kit quantitatively measures Sm/RNP IgG antibodies in human serum and plasma samples. Samples, calibrator, and controls are added to the wells pre-coated with Sm/RNP antigen. IgG antibodies to Sm/RNP, if present in the samples, will bind to the Sm/RNP antigen. The wells are then washed with Wash Buffer, followed by incubation with the enzyme conjugate. After incubation, any unattached conjugates are washed off by Wash Buffer. The enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is directly proportional to the amount of IgG antibodies to Sm/RNP present in the sample.

Assay Information

Features & Benefits

• Designed for quantitative measurement of Sm/RNP IgG.
• Sample type: Plasma; Serum.
• Detection method: Absorbance (450 nm).
• Kit size: 96 Well.

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Product Details