TRC-00707 | Sarafloxacin ELISA Kit | 96 Well
Product Overview
A competitive ELISA kit for the quantitative measurement of Sarafloxacin in Tissue, Honey, Milk (liquid/powder), Egg, Urine.
Sarafloxacin is a veterinary fluoroquinolone antimicrobial agent that inhibits bacterial DNA gyrase. It has been used for the control of bacterial infections in poultry caused by either E. coli or Salmonella species. Norfloxacin ELISA Kit is based on Competitive ELISA principle. The micro-plate provided in this kit has been pre-coated with Sarafloxacin. During the reaction, Sarafloxacin in the samples or standard competes with Sarafloxacin coated on the plate for binding to the anti-Sarafloxacin antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of Sarafloxacin. The concentration of Sarafloxacin in the samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Information
| Assay Format | Competitive ELISA |
| Species Reactivity | Universal |
| Sample Type | Tissue, Honey, Milk (liquid/powder), Egg, Urine |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Sensitivity | 0.1 ppb (ng/mL) |
| Detection Limit | Tissue (chicken, pork, fish, shrimp) - 0.3 ppb, Honey - 0.4 ppb, Milk - 3 ppb, Milk powder- 6 ppb, Egg - 3 ppb, Urine - 0.5 ppb, Sample recovery rate: Tissue, Honey, Milk, Milk powder, Egg- 85%±15%, Cross-reactivity: Sarafloxacin -100%,, Sensitivity: 0.1 ppb (ng/mL) |
Applications
This ELISA kit is used for quantitative measurement of Sarafloxacin in Tissue, Honey, Milk (liquid/powder), Egg, Urine.
Features & Benefits
- Designed for quantitative measurement of Sarafloxacin.
- Assay format: Competitive ELISA.
- Sample type: Tissue, Honey, Milk (liquid/powder), Egg, Urine.
- Detection method: Absorbance (450 nm).
- Sensitivity: 0.1 ppb (ng/mL).
- Kit size: 96 Well.
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 12 Months |
Product Details
| Catalog Number | TRC-00707 |
| Analyte / Target | Sarafloxacin |
| Alternate Name | 3110 |