TRC-00693 | Ribavirin ELISA Kit | 96 Well
Product Overview
A competitive ELISA kit for the quantitative measurement of Ribavirin in Tissue, Milk, Egg.
Ribavirin is a guanosine analog and an anti-viral agent that interferes with viral mRNA synthesis and capping. Upon activation by adenosine kinase, ribavirin triphosphate binds to the nucleotide-binding site of the viral mRNA polymerase, thereby preventing the binding of correct nucleotides and causing decreased viral replication and formation of defective virions. Ribavirin also inhibits viral mRNA guanylyltransferase and 2′-O-methyltransferase, thereby disrupting the posttranslational capping of the viral mRNA. The drug is recommended to treat hepatitis C and viral hemorrhagic fevers. 96Trace’s Ribavirin ELISA kit is used to quantitatively measure Ribavirin in muscle tissue, milk, and egg samples. The kit is based on the Competitive ELISA principle. Samples and standards are added to the microwell plate that is pre-coated with an antigen and competes for binding to the anti-Ribavirin antibody. The HRP conjugate is added to each well and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of substrate reagents. Finally, the reaction is terminated with an acidic stop solution. The color developed is inversely proportional to the concentration of Ribavirin in the samples.
Applications
This ELISA kit is used for quantitative measurement of Ribavirin in Tissue, Milk, Egg.
