TRC-00679 | Ranibizumab (Human) ELISA Kit | 96 Well
Product Overview
A sandwich ELISA kit for the quantitative measurement of Ranibizumab in Aqueous humor.
Ranibizumab is a recombinant humanized IgG1 monoclonal antibody fragment that targets human vascular endothelial growth factor A (VEGF-A). The antibody lacks an Fc-region and is smaller than the full-sized antibody, thus allowing efficient penetration into the retina and choroid. It inhibits the interaction of VEGF-A with its ligands on the surface of endothelial cells and thereby prevents neovascularization of the choroid. The antibody has been approved to treat wet age-related macular degeneration, retinal vein occlusion, and diabetic retinopathy. Ranibizumab ELISA kit has been developed for specific quantification of Ranibizumab concentration in human aqueous humor samples with high sensitivity and reproducibility. The kit is based on the sandwich ELISA principle. Standards and samples (aqueous humor) are added in the microtiter plate coated with the reactant for Ranibizumab. After incubation, the wells are washed. The HRP conjugated probe is added and binds to Ranibizumab captured by the reactant on the surface of the wells. Following incubation, wells are washed and the bound enzymatic activity is detected by the addition of TMB chromogen substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of Ranibizumab in the sample or standard. The results of samples can be determined directly using the standard curve.
Assay Information
| Assay Format | Sandwich ELISA |
| Species Reactivity | Human |
| Sample Type | Aqueous humor |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 0.3-30 ng/ml,Cross Reactivity: Except for Ranibizumab, there is no cross reaction with other therapeutic antibodies and native aqueous humor immunoglobins,Sensitivity: 0.3 ng/ml,For in vitro quantitative determination of Ranibizumab in human aqueous humor samples |
| Sensitivity | 0.3 ng/ml,For in vitro quantitative determination of Ranibizumab in human aqueous humor samples |
Applications
This ELISA kit is used for quantitative measurement of Ranibizumab in Aqueous humor.
Features & Benefits
- Designed for quantitative measurement of Ranibizumab.
- Assay format: Sandwich ELISA.
- Sample type: Aqueous humor.
- Detection method: Absorbance (450 nm).
- Assay Precision: Intra-Assay and Inter-Assay CV < 30%,Detection Range: 0.3-30 ng/ml,Cross Reactivity: Except for Ranibizumab, there is no cross reaction with other therapeutic antibodies and native aqueous humor immunoglobins,Sensitivity: 0.3 ng/ml,For in vitro quantitative determination of Ranibizumab in human aqueous humor samples
- Sensitivity: 0.3 ng/ml,For in vitro quantitative determination of Ranibizumab in human aqueous humor samples.
- Kit size: 96 Well.
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 12 Months |
Product Details
| Catalog Number | TRC-00679 |
| Analyte / Target | Ranibizumab |