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TRC-00676 | Quinolone ELISA Kit | 96 Well

Product Overview

A competitive ELISA kit for the quantitative measurement of Quinolone in serum, urine and milk.

Quinolones are one of the most commonly prescribed classes of antibiotics used to treat various bacterial infections in humans and domestic animals. Quinolones that contain additional fluorine atom in the core structure are called fluoroquinones. Both of these antibiotics can kill bacteria by inhibiting DNA gyrase in gram-negative bacteria or topoisomerase IV in gram-positive bacteria. However, excessive quinolone or fluoroquinolone in foods or drinks can pose a serious threat to humans as it may cause central nervous system toxicity, blood disorders, tendonitis, brain, liver and gastrointestinal dysfunction. As a consequence, it is important to monitor their levels in food products such as in meat and milk. The traditional techniques/instruments (HPLC or GC-MS) for detecting quinolone are complex, expensive, laborious, and time-consuming. Immunoassay techniques, such as ELISAs, are commonly preferred as a simple, reliable and rapid method. 96Trace’s Quinolone ELISA Kit is a competitive-based ELISA that can be used to detect both quinolone and fluoroquinolone in tissues, urine and serum. This detection kit offers ready-to-use reagents, and can quantify quinolone (0.12 – 10 ng/ml) within 90 minutes.

Assay Information

Assay FormatCompetitive ELISA
Species ReactivityUniversal
Sample TypeSerum, urine and milk
Detection MethodAbsorbance (450 nm)
Detection Wavelength450 nm
Kit Size96 Well

Performance Data

Detection Range0.12 - 10 ppb (ng/ml)
Sensitivity0.12 ppb
Detection Limit1 ppb (serum), 2 ppb (milk and urine)
Assay Time90 minutes

Applications

This ELISA kit is used for quantitative measurement of Quinolone in serum, urine and milk

Features & Benefits

  • Designed for quantitative measurement of Quinolone.
  • Assay format: Competitive ELISA.
  • Sample type: Serum, urine and milk.
  • Detection method: Absorbance (450 nm).
  • Detection Range: 0.12 - 10 ppb (ng/ml)
  • Sensitivity: 0.12 ppb
  • Assay Time: 90 minutes.
  • Kit size: 96 Well.

Storage & Shipping

Storage-20°C
ShippingGel Pack
Shelf Life12 Months

Product Details

Catalog NumberTRC-00676
Analyte / TargetQuinolone