TRC-00640 | Presepsin (Human) ELISA Kit | 96 Well
Product Overview
A Sandwich ELISA kit for the qualitative detect Presepsin in human serum or plasma.
Presepsin is a soluble subtype CD14. It is considered a novel biomarker for sepsis diagnostic and prognosis evaluation. 96Trace’s Presepsin ELISA kit is a sandwich ELISA assay for the quantitative measurement of Presepsin in human serum, plasma and cell culture supernatants. Anti-Presepsinantibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Presepsin antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Presepsin amount of sample captured in plate.
Assay Information
| Assay Format | Sandwich ELISA |
| Species Reactivity | Human |
| Sample Type | Plasma,Serum,Other biological fluids,Tissue and cell lysates |
| Detection Method | Colormetric |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 0.156-10 ng/ml |
| Sensitivity | < 0.094 ng/ml,Assay Precision: Intra-Assay: CV < 8% |
Applications
in vitro quantitative determination of Presepsin in human samples.
Features & Benefits
Easy, convenient and time-saving method to measure the level of Presepsin in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.,No significant cross-reactivity or interference was observed,Sensitivity: < 0.094 ng/ml,Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10%,Detection Range: 0.156-10 ng/ml
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 6 Months |
Product Details
| 96Trace CAT | E4696-100 |
| Supplier CAT | TRC-00640 |
| Analyte / Target | Presepsin |
| Category | Cardiovascular ELISA Kits |
| Alternate Name | sCD14 ELISA Kit,soluble CD14 subtype ELISA Kit,Presepsin ELISA Kit |