TRC-00630 | PGFM ELISA Kit | 96 Well
Product Overview
A competitive ELISA kit for the quantitative measurement of PGFM in Fecal Extracts,Plasma,Cell and tissue culture supernatants,Serum,Urine.
In many species, uterine and placental Prostaglandin F2alpha (PGF2a) is involved in the regulation of reproductive and pregnancy-related processes such as embryonic development, initiation of parturition, and resumption of ovarian activity. PGFM has been suggested as a useful non-invasive marker of pregnancy when measured in both urine and fecal samples. 96Trace’s PGFM ELISA kit is a competitive ELISA assay for the quantitative measurement of PGFM in fecal extracts, urine, serum and plasma samples. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. A PGFM-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to PGFM to each well. After incubation the plate is washed and substrate is added. The substrate reacts with the bound PGFM -peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength.
Applications
This ELISA kit is used for quantitative measurement of PGFM in Fecal Extracts,Plasma,Cell and tissue culture supernatants,Serum,Urine.
