TRC-00626 | PER1 (Human) ELISA Kit | 96 Well
Product Overview
A sandwich ELISA kit for the quantitative measurement of PER1 in Serum; Plasma; Other biological fluids; Tissue lysate.
Period circadian protein homolog 1 (PER1) is a master regulator of the circadian clock that belongs to the period family of proteins. It is expressed in almost all the cells of peripheral tissues, the most notable expression being in the suprachiasmatic nucleus (SCN), a primary circadian pacemaker in the brain. PER1 is involved in a transcription-translation feedback loop wherein it is activated by CLOCK-BMAL1 heterodimer inside the nucleus. PER1 then heterodimerizes with CRYs in the cytoplasm and reenters the nucleus to inhibit CLOCK-BMAL1 transcriptional activity. Besides playing a critical role in the entrainment of the circadian clock to the light-dark cycles, PER1 also regulates the cell cycle and response to DNA damage. Aberrant expression or altered rhythms of PER1 were associated with multiple cancers such as prostate, colon, breast, head and neck, and endometrial cancer. 96Trace’s PER1 (Human) ELISA kit quantitatively measures PER1 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. HRP-Streptavidin is added to the plate, and post-incubation any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of PER1 in the sample or standard.
Assay Information
| Assay Format | Sandwich ELISA |
| Species Reactivity | Human |
| Sample Type | Serum Plasma Other biological fluids Tissue lysate |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 15.625 – 1000 pg/ml |
| Sensitivity | 9.375 pg/ml |
Applications
This ELISA kit is used for quantitative measurement of PER1 in Serum; Plasma; Other biological fluids; Tissue lysate.
Features & Benefits
- Designed for quantitative measurement of PER1.
- Assay format: Sandwich ELISA.
- Sample type: Serum; Plasma; Other biological fluids; Tissue lysate.
- Detection method: Absorbance (450 nm).
- Detection range: 15.625 – 1000 pg/ml
- Sensitivity: 9.375 pg/ml
- Kit size: 96 Well.
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 6 Months |
Product Details
| Catalog Number | TRC-00626 |
| Analyte / Target | PER1 |
| Alternate Name | PER, hPER, RIGUI, PER1, HPER1 |