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TRC-00609 | Oxytetracycline ELISA Kit | 96 Well

Product Overview

A competitive ELISA kit for the quantitative measurement of Oxytetracycline in Tissue (muscle), Milk, Egg, Feed.

Oxytetracycline is a broad-spectrum antibiotic that belongs to the class of tetracyclines. It is effective against bacterial, chlamydial, and mycoplasma infections. The mechanism of action of this drug is to bind to 30S ribosomal subunit and prevent initiation of translation by binding of aminoacyl tRNA to the A site of the ribosome, thereby inhibiting protein synthesis. The drug can be used as a feed additive for food-producing animals to treat bacterial infections. It can also be used to promote growth in food-producing animals, promote milk and egg production and improve the efficiency of feed. 96Trace’s Oxytetracycline ELISA kit is used to quantitatively measure Oxytetracycline in tissue, milk, eggs, and feed samples. The kit is based on the Competitive ELISA principle. Samples and standards are added to the microwell plate that is pre-coated with an antigen and competes for binding to the anti-Oxytetracycline antibody. The HRP conjugate is added to each well and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of substrate reagents. Finally, the reaction is terminated with an acidic stop solution. The color developed is inversely proportional to the concentration of Oxytetracycline in the samples.

Assay Information

Assay FormatCompetitive ELISA
Species ReactivityUniversal
Sample TypeTissue (muscle), Milk, Egg, Feed
Detection MethodAbsorbance (450 nm)
Detection Wavelength450 nm
Kit Size96 Well

Performance Data

Sensitivity0.3ppb, Cross reaction: Oxytetracycline 100%, Chlortetracycline 300%, Tetracycline 206%, Doxycycline 18.5%, This ELISA kit is used for in vitro quantitative determination of Oxytetracycline
Detection Limit6ppb for muscle, milk, egg, 400ppb for feed, Sensitivity: 0.3ppb, Cross reaction: Oxytetracycline 100%, Chlortetracycline 300%, Tetracycline 206%, Doxycycline 18.5%, This ELISA kit is used for in vitro quantitative determination of Oxytetracycline

Applications

This ELISA kit is used for quantitative measurement of Oxytetracycline in Tissue (muscle), Milk, Egg, Feed.

Features & Benefits

  • Designed for quantitative measurement of Oxytetracycline.
  • Assay format: Competitive ELISA.
  • Sample type: Tissue (muscle), Milk, Egg, Feed.
  • Detection method: Absorbance (450 nm).
  • Sensitivity: 0.3ppb, Cross reaction: Oxytetracycline 100%, Chlortetracycline 300%, Tetracycline 206%, Doxycycline 18.5%, This ELISA kit is used for in vitro quantitative determination of Oxytetracycline.
  • Kit size: 96 Well.

Storage & Shipping

Storage+4°C
ShippingGel Pack
Shelf Life12 Months

Product Details

Catalog NumberTRC-00609
Analyte / TargetOxytetracycline
Alternate NameOxytetracycline