Osteopontin (OPN) (Human) ELISA Kit

Product Overview

A Sandwich ELISA kit to quantitatively measure Osteopontin (OPN) in human serum, plasma, tissue lysates, and other biological fluids

Osteopontin (OPN) is a 44 kDa phosphorylated acidic glycoprotein that is expressed in a variety of tissues such as bone, lungs, kidney, placenta, kidneys, immune cells, and epithelial cells of the gastrointestinal tract. It exists both as a component of the extracellular matrix as well as a soluble cytokine. OPN plays an important role in a variety of physiological functions such as bone mineralization, calcification, wound healing, and inflammatory responses. Elevated levels of OPN are associated with multiple pathological conditions such as osteoporosis, multiple sclerosis, Alzheimer's, kidney disease, inflammatory diseases such as inflammatory bowel disease, allergy, asthma, and cancers such as lung, breast, colorectal, and ovarian. Thus, OPN could serve as a potential therapeutic target for the treatment of human diseases. 96Trace’s Osteopontin (OPN) (Human) ELISA kit is based on the Sandwich ELISA principle to quantitatively measure the concentration of Osteopontin in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the concentration of Osteopontin in the sample or standard.

Assay Information

Performance Data

Applications

This Sandwich ELISA kit is designed to quantitatively measure amount of Osteopontin in human serum, plasma, tissue lysates, and other biological fluids

Features & Benefits

Sensitivity: 0.094 ng/ml,Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%,This Sandwich ELISA is highly sensitive and highly specific for the detection of Osteopontin in human samples. There is no significant cross-reactivity or interference between Osteopontin and analogues,Recovery range: 85 - 105% for normal human serum and plasma samples,Detection range: 0.156 - 10 ng/ml

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Product Details