Oncostatin-M (Human) ELISA Kit

Product Overview

A Sandwich ELISA kit for the quantitative measurement of Oncostatin-M in human serum, plasma, tissue homogenates, and other biological fluids

Oncostatin-M (OSM) is a pleiotropic cytokine that belongs to the family of IL-6 cytokines. OSM is produced by activated T-cells, dendritic cells, and several other cell types. It regulates cytokine production, inhibits adipocyte differentiation, and promotes differentiation of osteoblasts of mesenchymal stem cells. Additionally, OSM also regulates central nervous system development and tumorigenesis. 96Trace’s Oncostatin-M (Human) ELISA kit uses Sandwich ELISA principle to quantitatively measure the amount of OSM in human serum, plasma, and other biological fluids. Test Samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and subsequently washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed away with Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of OSM in the sample or standard.

Assay Information

Performance Data

Applications

This Sandwich ELISA kit is designed to quantitatively measure amount of Oncostatin-M in human serum, plasma, tissue lysates and other biological fluids

Features & Benefits

This Sandwich ELISA is highly sensitive and highly specific for the detection of OSM in human samples. There is no significant cross-reactivity or interference between OSM and analogues,Sensitivity: 9.375 pg/ml,Detection range: 15.625 - 1000 pg/ml,Recovery range: between 85 - 105% for normal human serum and plasma samples,Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%

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Product Details