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TRC-00590 | NOS3 / eNOS (Rat) ELISA Kit | 96 Well

Product Overview

A sandwich ELISA kit for the quantitative measurement of NOS3 / eNOS in Serum, plasma, tissue homogenates and other biological fluids.

Nitric oxide synthases (NOS) are a family of enzymes that catalyze the production of nitric oxide (NO) from L-arginine. Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. In presence of NADPH, FAD, FMN, (6R)-5,6,7,8-tetrahydrobiopterin, calmodulin and heme, NOS catalyzes a five-electron oxidation of the guanidino nitrogen of L-arginine with molecular oxygen to generate NO and L-citrulline. There are three isoforms of NOS: endothelial (eNOS), neuronal (nNOS), and inducible (eNOS). eNOS produces NO in blood vessels and is involved in regulation of vascular function. 96Trace’s NOS3 ELISA kit is a sandwich ELISA assay for the quantitative measurement of NOS3 in mouse serum, plasma and cell culture supernatants. The density of color is proportional to the amount of NOS3 captured from the samples.

Assay Information

Assay FormatSandwich ELISA
Species ReactivityRat
Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
Detection MethodAbsorbance (450 nm)
Detection Wavelength450 nm
Kit Size96 Well

Performance Data

Detection Range15.625 - 1000 pg/ml
Sensitivity< 9.375 pg/ml

Applications

This ELISA kit is used for quantitative measurement of NOS3 in serum, plasma, tissue homogenates and other biological fluids.

Features & Benefits

  • Designed for quantitative measurement of NOS3 / eNOS.
  • Assay format: Sandwich ELISA.
  • Sample type: Serum, plasma, tissue homogenates and other biological fluids.
  • Detection method: Absorbance (450 nm).
  • Detection Range: 15.625 - 1000 pg/ml
  • Sensitivity: < 9.375 pg/ml
  • Kit size: 96 Well.

Storage & Shipping

Storage+4°C
ShippingGel Pack
Shelf Life6 Months

Product Details

Catalog NumberTRC-00590
Analyte / TargetNOS3 / eNOS