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TRC-00586 | NOS2 / iNOS (mouse) ELISA Kit | 96 Well

Product Overview

A sandwich ELISA kit for the quantitative measurement of NOS2 / iNOS in Plasma,Serum.

Nitric oxide synthases (NOS) are a family of enzymes that catalyze the production of nitric oxide (NO) from L-arginine. Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. In presence of NADPH, FAD, FMN, (6R)-5,6,7,8-tetrahydrobiopterin, calmodulin and heme, NOS catalyzes a five-electron oxidation of the guanidino nitrogen of L-arginine with molecular oxygen to generate NO and L-citrulline. There are three isoforms of NOS: endothelial (eNOS), neuronal (nNOS), and inducible (iNOS). iNOS is expressed de novo under oxidative stress conditions and produces large amounts of NO as a part of body’s defense mechanism. 96Trace’s NOS2 ELISA kit is a sandwich ELISA assay for the quantitative measurement of NOS2 in mouse serum, plasma and cell culture supernatants. The density of color is proportional to the amount of NOS2 captured from the samples.

Assay Information

Assay FormatSandwich ELISA
Species ReactivityMouse
Sample TypePlasma,Serum
Detection Methodcolormetric
Detection Wavelength450 nm
Kit Size96 Well

Performance Data

Detection Range0.312-20 ng/ml,Assay Precision: Intra-Assay: CV < 8%
Sensitivity< 0.188 ng/ml,Detection method- Absorbance (450 nm),Detection Range: 0.312-20 ng/ml,Assay Precision: Intra-Assay: CV < 8%

Applications

This ELISA kit is used for quantitative measurement of NOS2 / iNOS in Plasma,Serum.

Features & Benefits

  • Designed for quantitative measurement of NOS2 / iNOS.
  • Assay format: Sandwich ELISA.
  • Sample type: Plasma,Serum.
  • Detection method: colormetric.
  • Detection Range: 0.312-20 ng/ml,Assay Precision: Intra-Assay: CV < 8%.
  • Sensitivity: < 0.188 ng/ml,Detection method- Absorbance (450 nm),Detection Range: 0.312-20 ng/ml,Assay Precision: Intra-Assay: CV < 8%.
  • Kit size: 96 Well.

Storage & Shipping

Storage+4°C
ShippingGel Pack
Shelf Life6 Months

Product Details

Catalog NumberTRC-00586
Analyte / TargetNOS2 / iNOS