Neuregulin-1/NRG1-beta1 (human) ELISA Kit

Product Overview

A sandwich ELISA kit for the quantitative measurement of Neuregulin-1/NRG1-beta1 in • Serum and plasma; • Cell culture supernatant.

96Trace’s human NRG-1 ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A mouse monoclonal antibody specific for human NRG-1 is coated on a 96-well plate. Standards and test samples are added to the wells and NRG-1 present in a sample is bound by the immobilized antibody. A biotinylated polyclonal detection antibody from goat specific for NRG-1 is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, HRP conjugate is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is used by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human NRG-1 captured onto the plate. This ELISA kit shows no detectable cross-reactivity with related proteins. Detection Range: 62.5 pg/ml – 4000 pg/ml. Sensitivity: < 10 pg/ml. Coefficient of variation: 5% (Intra-assay) and 7% (Inter-assay).

Assay Information

Performance Data

Applications

This ELISA kit is used for quantitative measurement of Neuregulin-1/NRG1-beta1 in • Serum and plasma; • Cell culture supernatant.

Features & Benefits

• Designed for quantitative measurement of Neuregulin-1/NRG1-beta1.
• Assay format: Sandwich ELISA.
• Sample type: • Serum and plasma; • Cell culture supernatant.
• Detection method: Absorbance (450 nm).
• Detection Range: 62.5 pg/ml – 4000 pg/ml.
• Sensitivity: <10 pg/ml. • Detection range: 62.5 pg/ml – 4000 pg/ml.
• Kit size: 96 Well.

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Product Details