TRC-00553 | MMP-2 (Rat) ELISA Kit | 96 Well
Product Overview
A sandwich ELISA kit for in vitro quantitative determination of rat MMP-2 concentrations in serum, plasma and other biological fluids.
The mammalian Matrix metalloproteinases (MMPs) degrade extracellular matrix in physiological and pathological processes. After cleavage of a single peptide domain of about 20 amino acids, the MMPs are secreted in latent forms. Upon activation, the N-terminal propeptide domain is cleaved to generate the active forms of MMP. MMP-2 (72 kDa type IV collagenase, Gelatinase-A) contains the basic structure of propeptide, catalytic, and hemopexin domains. It is an important proteinase in tissue remodeling. 96Trace’s MMP-2 ELISA kit is based on the Sandwich-ELISA principle. The micro ELISA plate provided in the kit has been pre-coated with an antibody specific to rat MMP-2. On addition of standards or samples to the micro ELISA plate, they react with Biotinylated detection antibody specific for rat MMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate, which on the addition of substrate gives blue color. The enzyme-substrate reaction is terminated by the addition of stop solution to turn the reaction yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat MMP-2.The concentration of Rat MMP-2 in the samples was calculated by comparing the OD of the samples to the standard curve.
Assay Information
| Assay Format | Sandwich ELISA |
| Species Reactivity | Rat |
| Sample Type | Plasma, Serum, Other biological fluids |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 0.31—20 ng/mL, in vitro quantitative determination of Rat MMP-2 concentrations in serum, plasma and other biological fluids., Sensitivity: 0.19 ng/mL |
| Sensitivity | 0.19 ng/mL |
Applications
Sensitivity: 0.19 ng/mL, Detection Range: 0.31—20 ng/mL, Specificity: This kit recognizes Rat MMP-2 in samples. No significant cross-reactivity or interference between Rat MMP-2 and analogues was observed., in vitro quantitative determination of Rat MMP-2 concentrations in serum, plasma and other biological fluids.
Features & Benefits
Specificity: This kit recognizes Rat MMP-2 in samples. No significant cross-reactivity or interference between Rat MMP-2 and analogues was observed., Detection Range: 0.31—20 ng/mL, in vitro quantitative determination of Rat MMP-2 concentrations in serum, plasma and other biological fluids., Sensitivity: 0.19 ng/mL
Storage & Shipping
| Storage | -20°C |
| Shipping | Gel Pack |
| Shelf Life | 6 Months |
Product Details
| 96Trace CAT | E4724-100 |
| Supplier CAT | TRC-00553 |
| Analyte / Target | MMP-2 |
| Category | Cancer Biomarker ELISA Kits |
| Alternate Name | MMP2, CLG4, CLG4A, MMP-II, MONA, TBE-1, Gelatinase A, Matrix Metalloproteinase 2 |