MMP-2 (Human) ELISA Kit

Product Overview

A sandwich ELISA kit for in vitro quantitative determination of human MMP-2 concentrations in serum, plasma and other biological fluids.

The mammalian Matrix metalloproteinases (MMPs) degrade extracellular matrix in physiological and pathological processes. After cleavage of a single peptide domain of about 20 amino acids, the MMPs are secreted in latent forms. Upon activation, the N-terminal propeptide domain is cleaved to generate the active forms of MMP. MMP-2 (72 kDa type IV collagenase, Gelatinase-A) contains the basic structure of propeptide, catalytic, and hemopexin domains. It is an important proteinase in tissue remodeling. 96Trace’s MMP-2 ELISA Kit is based on Sandwich ELISA principle. The micro ELISA plate provided in the kit has been pre-coated with an antibody specific to human MMP-2. On addition of standards or samples to the micro ELISA plate, they react with Biotinylated detection antibody specific for human MMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate, which on the addition of substrate gives blue color. The enzyme-substrate reaction is terminated by the addition of stop solution to turn the reaction yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human MMP-2. The concentration of Human MMP-2 in the samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Information

Performance Data

Applications

Coefficient of variation is < 10%., Detection Range: 0.78—50 ng/ml, Specificity: This kit recognizes Human MMP-2 in samples. No significant cross-reactivity or interference between Human MMP-2 and analogues was observed., A sandwich ELISA kit for in vitro quantitative determination of human MMP-2 concentrations in serum, plasma and other biological fluids., Sensitivity: 0.47 ng/ml

Features & Benefits

Coefficient of variation is < 10%., Specificity: This kit recognizes Human MMP-2 in samples. No significant cross-reactivity or interference between Human MMP-2 and analogues was observed., Detection Range: 0.78—50 ng/ml, Sensitivity: 0.47 ng/ml

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