TRC-00546 | MFN2 (Human) ELISA Kit | 96 Well
Product Overview
A Sandwich ELISA kit for quantitative measurement of MFN2 in human serum, plasma, tissue lysates, and other biological fluids
Mitofusin 2 (MFN2) is an outer mitochondrial membrane dynamin-like GTPase protein that participates in the fusion of mitochondria and ultimately affects the dynamics, distribution, quality control, and function of the mitochondria. MFN2 shares 80% sequence homology with MFN1, yet MFN2 is more versatile and is frequently associated with human diseases. MFN2 is critical for embryonic development. Studies in mice have demonstrated that deletion of MFN2 is lethal during midgestation, whereas ablation of MFN2 leads to abnormal development of the cerebellum. MFN2 has been implicated in the onset of several diseases such as cancer, Alzheimer’s disease, Parkinson’s disease, obesity/diabetes/insulin resistance, cardiomyopathies, and hence may be considered a promising therapeutic target. 96Trace’s MFN2 (Human) ELISA kit is based on the Sandwich principle to quantitatively measure MFN2 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is directly proportional to the concentration of MFN2 in the sample or standard.
Assay Information
| Assay Format | Sandwich ELISA |
| Species Reactivity | Human |
| Sample Type | Other biological fluids Tissue lysate Serum Plasma |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 31.25 – 2000 pg/ml |
| Sensitivity | 18.75 pg/ml |
Applications
Sandwich ELISA kit to quantitatively measure MFN2 in human serum, plasma, tissue lysate, and other biological fluids
Features & Benefits
- Sensitivity: 18.75 pg/ml
- Detection range: 31.25 – 2000 pg/ml
- Recovery range: 85 - 105% for normal human serum and plasma samples
- This Sandwich ELISA is highly sensitive and highly specific for the detection of MFN2 in human samples. There is no significant cross-reactivity or interference between MFN2 and analogues
- Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 6 Months |
Product Details
| 96Trace CAT | E4972-100 |
| Supplier CAT | TRC-00546 |
| Analyte / Target | MFN2 |
| Category | Neuroscience ELISA Kits |
| Alternate Name | HSG, MARF, CMT2A, CPRP1, CMT2A2, HMSN6A, CMT2A2A, CMT2A2B, MFN2 |