Metanephrine ELISA Kit

Product Overview

A competitive ELISA kit for the quantitative measurement of Metanephrine in Plasma, Serum, Other biological fluids.

Metanephrine is a metabolite of epinephrine created by action of catechol O-methyltransferase on epinephrine. Technically it is a product of epinephrine O-methylation. It is a commonly occurring, pharmacologically and physiologically inactive metabolite of epinephrine. This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Metanephrine. During the reaction, Metanephrine in the sample or standard competes with a fixed amount of Metanephrine on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to Metanephrine. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Metanephrine in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Information

Performance Data

Applications

This ELISA kit is used for quantitative measurement of Metanephrine in Plasma, Serum, Other biological fluids.

Features & Benefits

• Designed for quantitative measurement of Metanephrine.
• Assay format: Competitive ELISA.
• Sample type: Plasma, Serum, Other biological fluids.
• Detection method: Absorbance (450 nm).
• Detection Range: 0.16-10 ng/ml, Specificity: No Significant cross-reactivity or interference between MN and analogues was observed., Sensitivity: 0.10 ng/ml, Precision:Coefficient of variation is < 10%.
• Sensitivity: 0.10 ng/ml, Precision:Coefficient of variation is < 10%.
• Kit size: 96 Well.

Storage & Shipping

Product Details