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TRC-00512 | Leptin (Rat) ELISA Kit | 96 Well

Product Overview

A Sandwich ELISA kit for the quantitative measurement of Leptin in rat serum, plasma, culture supernatants and other biological fluids.

Leptin, the "satiety hormone", is a hormone made by adipose cells that helps to regulate energy balance by inhibiting hunger. Leptin is opposed by the actions of the hormone ghrelin, the "hunger hormone". Both hormones act on receptors in the arcuate nucleus of the hypothalamus to regulate appetite to achieve energy homeostasis. In obesity, a decreased sensitivity to leptin occurs, resulting in an inability to detect satiety despite high energy stores. 96Trace’s Leptin ELISA kit is a sandwich ELISA assay for the quantitative measurement of Leptin in rat serum, plasma and cell culture supernatants. The density of color is proportional to the amount of Leptin captured from the samples.

Assay Information

Assay FormatSandwich ELISA
Species ReactivityRat
Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
Detection MethodAbsorbance (450 nm)
Detection Wavelength450 nm
Kit Size96 Well

Performance Data

Detection Range0.156 - 10 ng/ml
Sensitivity< 0.094 ng/ml

Applications

This ELISA kit is used for quantitative measurement of Leptin in serum, plasma, tissue homogenates and other biological fluids.

Features & Benefits

  • Easy, convenient and time-saving method to measure the level of Leptin in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
  • Detection Range: 0.156 - 10 ng/ml
  • Sensitivity: < 0.094 ng/ml
  • Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10%
  • No significant cross-reactivity or interference was observed.

Storage & Shipping

Storage+4°C
ShippingGel Pack
Shelf Life6 Months

Product Details

96Trace CATE4655-100
Supplier CATTRC-00512
Analyte / TargetLeptin
CategoryCardiovascular ELISA Kits