TRC-00491 | Inositol Triphosphate (IP3) ELISA Kit | 96 Well
Product Overview
A competitive ELISA kit for the quantitative measurement of Inositol Triphosphate in Plasma, Serum, Other biological fluids.
Inositol trisphosphate receptors are Ca2+ channels that function to release Ca2+ from the endoplasmic reticulum in response to a wide array of hormones, growth factors, and neurotransmitters. Many fundamental biological processes that are activated or regulated by Ca2+ signals require IP3R function. These include such critical functions as secretion, smooth muscle contraction, gene transcription, and fertilization. Aberrant function of IP3Rs may contribute to the pathogenesis of several diseases. 96Trace’s IP3 ELISA Kit is based on Competitive ELISA principle. The micro-plate provided in this kit has been pre-coated with IP3. During the reaction, IP3 in the samples or standard competes with IP3 coated on the plate for binding to the anti-IP3 antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of IP3. The concentration of IP3 in the samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Information
| Assay Format | Competitive ELISA |
| Species Reactivity | Universal |
| Sample Type | Plasma, Serum, Other biological fluids |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 15.63-1000 pg/mL, Precision: Coefficient of variation is < 10%., Sensitivity: 9.38 pg/mL, Specificity: This kit recognizes IP3 in samples. No Significant cross-reactivity or interference between IP3 and analogues was observed |
| Sensitivity | 9.38 pg/mL, Specificity: This kit recognizes IP3 in samples. No Significant cross-reactivity or interference between IP3 and analogues was observed |
Applications
This ELISA kit is used for quantitative measurement of Inositol Triphosphate in Plasma, Serum, Other biological fluids.
Features & Benefits
- Designed for quantitative measurement of Inositol Triphosphate.
- Assay format: Competitive ELISA.
- Sample type: Plasma, Serum, Other biological fluids.
- Detection method: Absorbance (450 nm).
- Detection Range: 15.63-1000 pg/mL, Precision: Coefficient of variation is < 10%., Sensitivity: 9.38 pg/mL, Specificity: This kit recognizes IP3 in samples. No Significant cross-reactivity or interference between IP3 and analogues was observed.
- Sensitivity: 9.38 pg/mL, Specificity: This kit recognizes IP3 in samples. No Significant cross-reactivity or interference between IP3 and analogues was observed.
- Kit size: 96 Well.
Storage & Shipping
| Storage | An unopened kit can be stored at 2-8°C for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received |
| Shipping | Gel Pack |
| Shelf Life | 12 Months |
Product Details
| Catalog Number | TRC-00491 |
| Analyte / Target | Inositol Triphosphate |
| Alternate Name | Inositol trisphosphate, inositol 1, 4, 5-trisphosphate, triphosphoinositol, InsP3, Ins3P, IP3 |