TRC-00478 | IL-33 (Human) ELISA Kit | 96 Well
Product Overview
A Sandwich ELISA kit to quantitatively measure IL-33 in human serum, plasma, tissue lysates, and other biological fluids
Interleukin-33 (IL-33) belongs to the family of IL-1 cytokines. It is constitutively expressed at increased levels in the nuclei of multiple cell types such as glial, endothelial, smooth muscle, and immune cells. When present in the nucleus, IL-33 functions as a transcriptional repressor, whereas extracellularly, it acts as a traditional cytokine by inducing Th2-type immunity. It also acts as an ‘alarmin’ for the immune system when released by damaged tissues or cell necrosis. Expression of IL-33 is upregulated in cancer and immune-related diseases such as rheumatoid arthritis, asthma, psoriatic arthritis. IL-33 also plays a protective role in cardiovascular and neurodegenerative diseases. Hence, IL-33 could potentially serve as an efficient biomarker for human diseases. 96Trace’s IL-33 (Human) ELISA kit is based on the Sandwich ELISA principle to quantitatively measure the amount of IL-33 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and subsequently washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of IL-33 in the sample or standard.
Assay Information
| Assay Format | Sandwich ELISA |
| Species Reactivity | Human |
| Sample Type | Plasma,Serum,Tissue lysate,Other biological fluids |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 15.625 - 1000 pg/ml,Recovery range: 85 - 105% for normal human serum and plasma samples,Assay Precision: Intra-Assay CV < |
| Sensitivity | 9.375 pg/ml,Detection range: 15.625 - 1000 pg/ml,Recovery range: 85 - 105% for normal human serum and plasma samples,Assay Precision: Intra-Assay CV < |
Applications
This Sandwich ELISA kit is designed to quantitatively measure amount of IL-33 in human serum, plasma, tissue lysates, and other biological fluids
Features & Benefits
This Sandwich ELISA is highly sensitive and highly specific for the detection of IL-33 in human samples. There is no significant cross-reactivity or interference between IL-33 and analogues,Sensitivity: 9.375 pg/ml,Detection range: 15.625 - 1000 pg/ml,Recovery range: 85 - 105% for normal human serum and plasma samples,Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 6 Months |
Product Details
| 96Trace CAT | E4888-100 |
| Supplier CAT | TRC-00478 |
| Analyte / Target | IL-33 |
| Category | Cancer Biomarker ELISA Kits |
| Alternate Name | Interleukin 33, Interleukin-1 Family Member 11, DVS27-Related Protein, Interleukin-33, DKFZp586H0523, IL-1F11, NFHEV, IL-33, DVS27, IL1F11, NF-HEV, NFEHEV, C9orf26, IL33 |