IL-1β (Human) ELISA Kit

Product Overview

A Sandwich ELISA kit for the quantitative measurement of IL-1β in human plasma and other biological fluids.

IL-1β is a potent proinflammatory cytokine. It promotes Th17 differentiation of T-cells and synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. 96Trace’s IL-1β (Human) ELISA kit is based on Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-1β. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Human IL-1β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of IL-1β bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human IL-1 β. The concentration of Human IL-1β in the samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Information

Performance Data

Applications

In vitro quantitative determination of IL-1β concentrations in human serum, plasma and other biological fluids.

Features & Benefits

Sensitivity: 4.69 pg/mL,Specificity: This kit recognizes Human IL-1β in samples. No Significant cross-reactivity or interference between Human IL-1β and analogues was observed.,Precision: Coefficient of variation is < 10%.,Detection Range: 7.81-500 pg/mL

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Product Details