TRC-00470 | IL-1α (Rat) ELISA Kit | 96 Well
Product Overview
A Sandwich ELISA kit for the quantitative measurement of IL-1α in rat serum, plasma and other biological fluids.
IL-1α is produced by activated macrophages. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. 96Trace’s IL-1α (Rat) ELISA Kit is based on the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-1α. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Rat IL-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of IL-1α bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat IL-1 α. The concentration of Rat IL-1α in the samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Information
| Assay Format | Sandwich ELISA |
| Species Reactivity | Rat |
| Sample Type | Plasma,Serum,Other biological fluids |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 15.63-1000 pg/mL,Sensitivity: 9.38 pg/mL,Specificity: No Significant cross-reactivity or interference between Rat IL-1α and analogues was observed |
| Sensitivity | 9.38 pg/mL,Specificity: No Significant cross-reactivity or interference between Rat IL-1α and analogues was observed |
Applications
in vitro quantitative measurement of IL-1α in rat serum, plasma and other biological fluids.
Features & Benefits
Repeatability: Coefficient of variation is < 10%.,Detection Range: 15.63-1000 pg/mL,Sensitivity: 9.38 pg/mL,Specificity: No Significant cross-reactivity or interference between Rat IL-1α and analogues was observed.
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 6 Months |
Product Details
| 96Trace CAT | E4804-100 |
| Supplier CAT | TRC-00470 |
| Analyte / Target | IL-1α |
| Category | Cytokine & Growth Factor ELISA Kits |
| Alternate Name | IL1A, IL1-A, IL1, IL1F1, Preinterleukin 1 Alpha, Hematopoietin-1, Pro-Interleukin-1-Alpha |