TRC-00450 | IGFBP-3 (Human) ELISA Kit | 96 Well
Product Overview
A sandwich ELISA kit for the quantitative measurement of IGFBP-3 in Plasma,Serum,Tissue lysate,Other biological fluids.
Insulin-like growth factor binding protein-3 (IGFBP-3) is a 264 amino acid peptide that belongs to the family of IGF-binding proteins. It is expressed in multiple tissues. One of the main functions of IGFBP-3 is that it regulates the transport, bioavailability, and half-life of insulin-like growth factors, especially IGF-1. IGFBP-3 is inversely correlated with cancer. Increased levels of IGFBP-3 in the serum inhibit proliferation and promote apoptosis of cancer cells, thus serving as a potential target for cancer research. 96Trace’s IGFBP-3 (Human) ELISA kit is based on the Sandwich ELISA principle to quantitatively measure the amount of IGFBP-3 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and subsequently washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off with Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of IGFBP-3 in the sample or standard.
Applications
This ELISA kit is used for quantitative measurement of IGFBP-3 in Plasma,Serum,Tissue lysate,Other biological fluids.
