His-tag Protein ELISA kit

Product Overview

A competitive ELISA kit for the quantitative measurement of His-tag Protein in bacterial, insect and mammalian cell lysates, downstream analyses of purification of His-tag proteins, His-tag proteins used in protein-protein interaction studies.

A His-tag is an amino acid motif that contains at least six consecutive histidine (His) residues, often at the N- or C-terminus of a protein. More than 50% of recombinant proteins expressed by eukaryotic or prokaryotic expression systems are tagged with His-tag. Selective, sensitive and quantitative detection of protein levels and protein-protein binding partners expressed with His-tag is therefore a valuable tool in Life Science research. 96Trace’s His-Tag Protein ELISA Kit is a competitive-based ELISA that is easier, faster and more sensitive method to detect His-tag proteins expressed in both bacterial, insect and mammalian cells. This detection kit offers ready-to-use reagents, and can detect as low as 20 ng/ml of His-tag protein within 90 min.

Assay Information

Performance Data

Applications

This ELISA kit is used for quantitative measurement of His-Tag Protein in bacterial, insect and mammalian cell lysates, downstream analyses of purification of His-tag proteins, His-tag proteins used in protein-protein interaction studies

Features & Benefits

• Designed for quantitative measurement of His-tag Protein.
• Assay format: Competitive ELISA.
• Sample type: bacterial, insect and mammalian cell lysates, downstream analyses of purification of His-tag proteins, His-tag proteins used in protein-protein interaction studies.
• Detection method: Absorbance (450 nm).
• Detection Range: 25.6 – 3200 ng/ml
• Sensitivity: 20 ng/ml
• Assay Time: 90 min.
• Kit size: 96 Well.

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Product Details