Skip to Content

TRC-00416 | HIF-1 alpha (Human) ELISA Kit | 96 Well

Product Overview

A sandwich ELISA kit for the quantitative measurement of HIF-1 alpha in Human serum, plasma, tissue homogenates and other biological fluids.

HIF-1 alpha functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia. 96Trace’s HIF-1 alpha ELISA kit is a sandwich ELISA assay for the quantitative measurement of human HIF-1 alpha in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of human HIF-1 alpha captured from the samples.

Assay Information

Assay FormatSandwich ELISA
Species ReactivityHuman
Sample TypeHuman serum, plasma, tissue homogenates and other biological fluids.
Detection MethodAbsorbance (450 nm)
Detection Wavelength450 nm
Kit Size96 Well

Performance Data

Detection Range0.156 – 10 ng/ml
Sensitivity< 0.094 ng/ml

Applications

This ELISA kit is used for quantitative measurement of HIF-1 alpha in human serum, plasma, tissue homogenates and other biological fluids.

Features & Benefits

  • Designed for quantitative measurement of HIF-1 alpha.
  • Assay format: Sandwich ELISA.
  • Sample type: Human serum, plasma, tissue homogenates and other biological fluids.
  • Detection method: Absorbance (450 nm).
  • Detection Range: 0.156 – 10 ng/ml
  • Sensitivity: < 0.094 ng/ml
  • Kit size: 96 Well.

Storage & Shipping

Storage+4°C
ShippingGel Pack
Shelf Life6 Months

Product Details

Catalog NumberTRC-00416
Analyte / TargetHIF-1 alpha