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TRC-00415 | Hexokinase (Rat) ELISA Kit | 96 Well

Product Overview

A sandwich ELISA kit for the quantitative measurement of Hexokinase in Serum, plasma, tissue homogenates and other biological fluids.

Hexokinases (HK) are found in many organisms including bacteria, plants and mammals and play an important role in glucose metabolism. Hexokinases phosphorylate glucose and generate glucose-6-phosphate for glycolysis. Hexokinases have four isoforms (HK-I, II, III and IV). HK-I, HK-II and HK-III have low Km, while HK-IV has 100 fold high Km. Hexokinase deficiency leads to severe human diseases such as X-linked muscular dystrophy and a rare autosomal recessive hemolytic anemia. On the other hand, increased hexokinase activity is detected in various human tumors and is associated with metastasis. Early detection of abnormal hexokinase activity is crucial for diagnosis, prediction and treatment of the disease. 96Trace’s Hexokinase ELISA kit is a sandwich ELISA assay for the quantitative measurement of Hexokinase in rat serum, plasma and cell culture supernatants. The density of color is proportional to the amount of Hexokinase captured from the samples.

Assay Information

Assay FormatSandwich ELISA
Species ReactivityRat
Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
Detection MethodAbsorbance (450 nm)
Detection Wavelength450 nm
Kit Size96 Well

Performance Data

Detection Range31.25 - 2000 pg/ml
Sensitivity< 18.75 pg/ml

Applications

This ELISA kit is used for quantitative measurement of hexokinase in rat serum, plasma, tissue homogenates and other biological fluids.

Features & Benefits

  • Designed for quantitative measurement of Hexokinase.
  • Assay format: Sandwich ELISA.
  • Sample type: Serum, plasma, tissue homogenates and other biological fluids.
  • Detection method: Absorbance (450 nm).
  • Detection Range: 31.25 - 2000 pg/ml
  • Sensitivity: < 18.75 pg/ml
  • Kit size: 96 Well.

Storage & Shipping

Storage+4°C
ShippingGel Pack
Shelf Life12 Months

Product Details

Catalog NumberTRC-00415
Analyte / TargetHexokinase