TRC-00402 | Heme Oxygenase 1 (HO1) (Human) ELISA Kit | 96 Well
Product Overview
A Sandwich ELISA kit for the quantitative measurement of Heme Oxygenase 1 in human serum, plasma, culture supernatants and other biological fluids.
Heme Oxygenase 1 (HO1) cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis. 96Trace’s HO1 ELISA kit is a sandwich ELISA assay for the quantitative measurement of HO1 in human serum, plasma and cell culture supernatants. The density of color is proportional to the amount of HO1 captured from the samples.
Assay Information
| Assay Format | Sandwich ELISA |
| Species Reactivity | Human |
| Sample Type | Serum, plasma, tissue homogenates and other biological fluids. |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 0.313 - 20 ng/ml |
| Sensitivity | < 0.188 ng/ml |
Applications
This ELISA kit is used for quantitative measurement of HO1 in human serum, plasma, tissue homogenates and other biological fluids.
Features & Benefits
- Easy, convenient and time-saving method to measure the level of HO1 in human serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
- Detection Range: 0.313 - 20 ng/ml
- Sensitivity: < 0.188 ng/ml
- Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10%
- This assay has high sensitivity and excellent specificity for detection of HO1. No significant cross-reactivity or interference between HO1 and analogues was observed.
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 6 Months |
Product Details
| 96Trace CAT | E4507-100 |
| Supplier CAT | TRC-00402 |
| Analyte / Target | Heme Oxygenase 1 (HO1) |
| Category | Cardiovascular ELISA Kits |