Gasdermin D (Mouse) ELISA Kit

Product Overview

A Sandwich ELISA kit for the quantitative measurement of mouse Gasdermin D in cell culture supernatant and cell lysate

The gasdermin family members contain N-terminal domains that are capable of forming membrane pores to induce cytolysis, whereas the C-terminal domains of gasdermins function as inhibitors of such cytolysis through intramolecular domain association. Caspase-1 or -11 cleavage of gasdermin D is required for regulation of pyroptosis: upon protease cleavage of the gasdermin N- and C-domain linker, the disruption of the intramolecular domain interaction in the presence of lipids releases the N-domain to assemble oligomeric membrane pores that trigger cell death. Gasdermin D seems to be a key effector in the LPS-induced lethal sepsis. 96Trace’s Gasdermin D (Mouse) ELISA Kit is based on the Sandwich-ELISA principle. An antibody specific for Gasdermin D has been precoated onto the 96-well microtiter plate. Standards (STD) and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, Gasdermin D is recognized by the addition of a detection antibody specific for Gasdermin D (C-terminus) (DET). After removal of excess antibody, HRP conjugated anti-Guinea Pig IgG (HRP) is added. Following a final washing, peroxidase activity is quantified using the TMB substrate. The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of mouse Gasdermin D in the samples.

Assay Information

Performance Data

Applications

in-vitro quantitative determination of mouse Gasdermin D in cell culture supernatant and cell lysate

Features & Benefits

Sensitivity: 14 pg/ml,Detection limit: 15.625 pg/ml - 1000 pg/ml,Specificity: This ELISA is specific for the measurement of natural and recombinant mouse Gasdermin D (fulllength and C-terminus cleaved fragment). It does not detect human Gasdermin D.

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