Epiandrosterone ELISA Kit

Product Overview

A competitive ELISA kit for the quantitative measurement of Epiandrosterone in Extracted Serum or Plasma,Cell and tissue culture supernatants,Dried Fecal Extracts,Urine,Saliva.

Epiandrosterone, or 3ß-androsterone, also known as 3ß-hydroxy-5a-androstan-17-one or 5α-androstan-3ß-ol17-one, is a steroid hormone with weak androgenic activity. It is a natural metabolite of dehydroepiandrosterone (DHEA) via the 5α-reductase enzyme. 96Trace’s Epiandrosterone ELISA kit is a competitive ELISA assay for the quantitative measurement of Epiandrosterone in urine, saliva, and fecal samples, or in extracted serum and plasma. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An Epiandrosterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to Epiandrosterone to each well. After incubation the plate is washed and substrate is added. The substrate reacts with the bound Epiandrosterone -peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength.

Assay Information

Performance Data

Applications

This ELISA kit is used for quantitative measurement of Epiandrosterone in Extracted Serum or Plasma,Cell and tissue culture supernatants,Dried Fecal Extracts,Urine,Saliva.

Features & Benefits

• Designed for quantitative measurement of Epiandrosterone.
• Assay format: Competitive ELISA.
• Sample type: Extracted Serum or Plasma,Cell and tissue culture supernatants,Dried Fecal Extracts,Urine,Saliva.
• Detection method: Absorbance (450 nm).
• Detection Range: 0.098 - 100 ng/mL.
• Sensitivity: 0.120 ng/mL.,This ELISA kit is used for in vitro quantitative determination of Epiandrosterone.,Detection Range: 0.098 - 100 ng/mL.
• Kit size: 96 Well.

Storage & Shipping

Product Details