TRC-00299 | Durvalumab (Human) ELISA Kit | 96 Well
Product Overview
A Sandwich ELISA kit for the Quantitative measurement of free Durvalumab in human serum or plasma samples
Durvalumab is a human IgG1 monoclonal antibody that binds to and inhibits programmed cell death ligand 1 (PD-L1). PD-L1 is an important immune checkpoint molecule expressed on tumor cells and tumor-infiltrating immune cells. PD-L1 binds to its receptors PD-1 and B7.1 located on the surface of activated T cells and antigen-presenting cells and in turn suppresses cytotoxic T-cell activity, the proliferation of T-cells, and production of cytokines. The monoclonal antibody binds to PD-L1 and inhibits its anti-tumor immune response, and thus reduces tumor growth without causing antibody-dependent cellular cytotoxicity. The original antibody is approved to treat locally advanced or metastatic urothelial carcinoma and locally advanced unresectable non-small cell lung cancer (NSCLC). Durvalumab ELISA kit measures free Durvalumab concentration in human serum or plasma with high sensitivity and reproducibility. The kit is based on the Sandwich ELISA principle. Standards and samples (serum or plasma) are added to the microtiter plate coated with the reactant for Durvalumab. After incubation, the wells are washed. The HRP conjugated probe is added and binds to Durvalumab captured by the reactant on the surface of the wells. Following incubation, wells are washed and the enzymatic activity is detected by the addition of TMB chromogen substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of Durvalumab in the sample or standard. The sample results can be determined directly using the standard curve.
Assay Information
| Assay Format | Sandwich ELISA |
| Species Reactivity | Human |
| Sample Type | Plasma,Serum |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 10 - 300 ng/ml,Cross Reactivity: Except for Durvalumab, there is no cross reaction with other therapeutic antibodies and native serum immunoglobins,Assay Precision: Intra-Assay and Inter-Assay CV < |
| Sensitivity | 10 ng/mL |
Applications
The Sandwich ELISA kit is used for in vitro Quantitative determination of free Durvalumab in human serum and plasma samples
Features & Benefits
For in vitro Quantitative determination of free Durvalumab in human serum and plasma samples,Recovery rate: < 100 ± 30% with known concentrations of normal human serum samples,Detection Range: 10 - 300 ng/ml,Cross Reactivity: Except for Durvalumab, there is no cross reaction with other therapeutic antibodies and native serum immunoglobins,Assay Precision: Intra-Assay and Inter-Assay CV < 30%,Sensitivity: 10 ng/mL
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 12 Months |
Product Details
| 96Trace CAT | E4925-100 |
| Supplier CAT | TRC-00299 |
| Analyte / Target | Durvalumab |
| Category | Biosimilar & Isotype Control ELISA Kits |
| Alternate Name | B7-H, B7H1, PDL1, hPD-L1, PDCD1L1, PDCD1LG1, CD274 Molecule, Programmed Cell Death 1 Ligand 1, CD274 Antigen, B7 Homolog 1, Programmed Death Ligand 1, PDCD1 Ligand 1, CD274, B7-H1, CAS 1428935-60-7 |