Dengue Virus IgG Capture ELISA Kit

Product Overview

A sandwich ELISA kit for the qualitative detection of Dengue Virus IgG Capture in Serum and plasma.

The mosquito-borne dengue viruses (serotype 1-4) cause dengue fever, a severe flu-like illness. The disease is prevalent in Third World tropical regions and spreading to sub-tropical developed countries - including the United States. WHO estimates that 50-80 million cases of dengue fever occur worldwide each year, including a potentially deadly form of the disease called dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). Primary infection with dengue virus results in a self-limiting disease characterized by mild to high fever lasting 3 to 7 days, severe headache with pain behind the eyes, muscle and joint pain, rash, and vomiting. Secondary infection is the more common form of the disease in many parts of Southeast Asia and South America. This form of the disease is more serious and can result in DHF and DSS. The major clinical symptoms can include high fever, hemorrhagic events, and circulatory failure, and the fatality rate can be as high as 40%. Early diagnosis of DSS is particularly important, as patients may die within 12 to 24 h if appropriate treatment is not administered. Primary dengue virus infection is characterized by elevations in specific IgM antibody levels 3 to 5 days after the onset of symptoms; this generally persists for 30 to 60 days. IgG levels also become elevated after 10 to 14 days and remain detectable for life. During secondary infection, IgM levels generally rise more slowly and reach lower levels than in primary infection, while IgG levels rise rapidly from 1 to 2 days after the onset of symptoms. 96Trace’s Dengue Virus IgG Capture ELISA kit enables to detect the IgG antibodies to the Dengue virus in human serum and plasma samples. Diluted human serum/plasma samples and anti-human IgG biotinylated antibody are added to the microwell plate pre-coated with Streptavidin. The IgG antibodies in the serum/plasma bind to the anti-human IgG antibody and this complex binds to the Streptavidin-coated plate. Unbound complexes are washed off using wash buffer. The enzyme conjugate is added and any IgG antibodies, if present, will bind to this enzyme conjugate. The color developed as a result of the enzyme-substrate reaction is directly proportional to the amount of IgG antibodies to the Dengue virus in the human serum/plasma samples.

Assay Information

Applications

This ELISA kit is used for qualitative detection of Dengue Virus IgG Capture in Serum and plasma.

Features & Benefits

• Designed for qualitative detection of Dengue Virus IgG Capture.
• Assay format: Sandwich ELISA.
• Sample type: Serum and plasma.
• Detection method: Absorbance (450 nm).
• Kit size: 96 Well.

Storage & Shipping

Product Details