CTGF (Rat) ELISA Kit

Product Overview

A Sandwich ELISA kit for the quantitative measurement of rat CTGF in serum, plasma and other biological fluids.

CTGF is a major connective tissue mitoattractant secreted by vascular endothelial cells. It promotes proliferation and differentiation of chondrocytes. It also mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells and enhances fibroblast growth factor-induced DNA synthesis. 96Trace’s CTGF (Rat) ELISA Kit is based on the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CTGF. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Rat CTGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of CTGF bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat CTGF. The concentration of Rat CTGF in the samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Information

Performance Data

Applications

A Sandwich ELISA kit for the quantitative measurement of rat CTGF in serum, plasma and other biological fluids.

Features & Benefits

Sensitivity: 18.75 pg/mL,Detection Range: 31.25-2000 pg/mL,Precision: Coefficient of variation is < 10%,Specificity: No Significant cross-reactivity or interference between Rat CTGF and analogues was observed.

Storage & Shipping

Product Details