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TRC-00253 | Creatine Kinase MB (CK-MB) (Human) ELISA Kit | 96 Well

Product Overview

A Sandwich ELISA kit for the quantitative measurement of Creatine Kinase MB in human serum, plasma, culture supernatants and other biological fluids.

Creatine Kinase (CK) also known as creatine phosphokinase (CPK) and ATP: creatine N-phosphotransferase is a common cellular enzyme. It catalyzes the reversible conversion of creatine and ATP into ADP and phosphocreatine. CK is widely expressed in various tissues and cell types, with highest activity in striated muscles, heart tissue and brain. CK consists of two subunits: M (muscle) and B (brain), and has three isoenzymes: CK-MM (skeleton muscle), CK-MB (cardiac muscle), and CK-BB (brain). Increased CK level is associated with many diseases such as myocardial infarction, muscular dystrophy, pulmonary infarction and brain tumors. Accurate measurement of CK is crucial for early diagnosis, prediction and therapeutic strategy. 96Trace’s CK-MB ELISA kit is a sandwich ELISA assay for the quantitative measurement of CK-MB in Human serum, plasma and cell culture supernatants. The density of color is proportional to the amount of CK-MB captured from the samples.

Assay Information

Assay FormatSandwich ELISA
Species ReactivityHuman
Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
Detection MethodAbsorbance (450 nm)
Detection Wavelength450 nm
Kit Size96 Well

Performance Data

Detection Range0.156 - 10 ng/ml
Sensitivity< 0.094 ng/ml

Applications

This ELISA kit is used for quantitative measurement of Creatine Kinase MB in human serum, plasma, tissue homogenates and other biological fluids.

Features & Benefits

  • Easy, convenient and time-saving method to measure the level of Creatine Kinase MB in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
  • Detection Range: 0.156 - 10 ng/ml
  • Sensitivity: < 0.094 ng/ml
  • Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10%
  • No significant cross-reactivity or interference was observed.

Storage & Shipping

Storage+4°C
ShippingGel Pack
Shelf Life6 Months

Product Details

96Trace CATE4606-100
Supplier CATTRC-00253
Analyte / TargetCreatine Kinase MB (CK-MB)
CategoryCardiovascular ELISA Kits