TRC-00249 | COX2 (Human) ELISA Kit | 96 Well
Product Overview
A Sandwich ELISA kit for the quantitative measurement of COX2 in human serum, plasma and other biological fluids.
Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. 96Trace’s COX2 (Human) ELISA kit is based on Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to human COX2. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for human COX2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of COX2 bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of human COX2. The concentration of human COX2 in the samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Information
| Assay Format | Sandwich ELISA |
| Species Reactivity | Human |
| Sample Type | Plasma, Serum, Other biological fluids |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 0.31-20 ng/mL, Precision: Coefficient of variation is < 10%., Sensitivity: 0.19 ng/mL |
| Sensitivity | 0.19 ng/mL |
Applications
in vitro quantitative measurement of COX2 in human serum, plasma and other biological fluids.
Features & Benefits
Specificity: No Significant cross-reactivity or interference between Human COX2 and analogues was observed., Detection Range: 0.31-20 ng/mL, Precision: Coefficient of variation is < 10%., Sensitivity: 0.19 ng/mL
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 12 Months |
Product Details
| 96Trace CAT | E4798-100 |
| Supplier CAT | TRC-00249 |
| Analyte / Target | COX2 |
| Category | Metabolism & Hormone ELISA Kits |
| Alternate Name | COX2, mitochondrially encoded cytochrome c oxidase II, COII, MTCO2, Cytochrome c oxidase subunit II, CO II |