TRC-00242 | Complement C3 (Human) ELISA Kit | 96 Well
Product Overview
A Sandwich ELISA kit for quantitative measurement of Complement C3 in human serum, plasma, tissue lysates, and other biological fluids
Complement C3 is a 187 kDa protein that belongs to the a2-macroglobulin (a2M) family of proteins. It plays an important role in the activation of the complement system. The presence of foreign microorganisms triggers the proteolytic cleavage of C3 by convertases to yield an anaphylatoxin C3a and an opsonizing agent C3b. Circulating levels of C3 in the blood are used as indicators to measure therapeutic efficacy in patients suffering from autoimmune diseases. Patients having homozygous deficiency of C3 suffer from severe bacterial infections. Reduced levels of C3 are associated with diseases such as acute glomerulonephritis, systemic lupus erythematosus, septic shock, and liver disease. 96Trace’s Complement C3 (Human) ELISA kit quantitatively measures C3 in human serum, plasma, and other biological fluids. The kit is based on the Sandwich ELISA principle. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is directly proportional to the concentration of C3 in the sample or standard.
Assay Information
| Assay Format | Sandwich ELISA |
| Species Reactivity | Human |
| Sample Type | Plasma Serum Tissue lysate Other biological fluids |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 7.813 – 500 ng/ml |
| Sensitivity | 4.688 ng/ml |
Applications
Sandwich ELISA kit to quantitatively measure Complement C3 in human serum, plasma, tissue lysate, and other biological fluids
Features & Benefits
- Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%
- Detection range: 7.813 – 500 ng/ml
- Sensitivity: 4.688 ng/ml
- This Sandwich ELISA is highly sensitive and highly specific for the detection of Complement C3 in human samples. There is no significant cross-reactivity or interference between Complement C3 and analogues
- Recovery range: 85 - 105% for normal human serum and plasma samples
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 6 Months |
Product Details
| 96Trace CAT | E4976-100 |
| Supplier CAT | TRC-00242 |
| Analyte / Target | Complement C3 |
| Category | Infectious Disease ELISA Kits |
| Alternate Name | ASP, C3a, C3b, AHUS5, ARMD9, CPAMD1, HEL-S-62p, Complement C3, C3 |