TRC-00228 | Cimaterol ELISA Kit | 96 Well
Product Overview
A Competitive ELISA kit for in vitro quantitation of Cimaterol concentrations in food samples
Cimaterol is a chemically stable nonselective agonist β1-, β2-, and β3-adrenoceptors. Cimaterol is used in sport as a stimulant and a fat burner that assists bodybuilders and strength athletes to get rid of body fats. Cimaterol has been used in farmed animals (swine, fowl, etc.) to increase carcass mass and to alter muscle and fat deposition. 96Trace’s Cimaterol ELISA Kit is based on the Competitive ELISA method. It can detect Cimaterol in Tissue, Urine and Feed samples. The microtiter plate provided in the kit has been pre-coated with Cimaterol. During the reaction, Cimaterol in the samples or standard competes with coated Cimaterol for anti- Cimaterol antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of Cimaterol. The concentration of Cimaterol in the samples can be calculated by comparing the OD of the samples to the standard curve.
Assay Information
| Assay Format | Competitive ELISA |
| Species Reactivity | Universal |
| Sample Type | Tissue lysate, Feed, Urine |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Sensitivity | 0.3 ppb (ng/mL) |
| Detection Limit | Urine - 0.3 ppb, Tissue (treatment method 1) - 1.2 ppb, Tissue (treatment method 2) - 0.3 ppb, Feed - 3 ppb., Sample recovery rate: Urine - 95%±10%, Tissue, Feed - 85%±15%, Cross-reactivity: Clenbuterol - 100%, Arubendol - <1%, Mabuterol - <1%, Brombuterol - <1%, Salbutamol - <1%, Ractopamine - <1%, Sensitivity: 0.3 ppb (ng/mL) |
Applications
in vitro quantitation of Cimaterol concentrations in Urine, Tissue, Feed samples
Features & Benefits
Detection limit: Urine - 0.3 ppb, Tissue (treatment method 1) - 1.2 ppb, Tissue (treatment method 2) - 0.3 ppb, Feed - 3 ppb., Sample recovery rate: Urine - 95%±10%, Tissue, Feed - 85%±15%, Cross-reactivity: Clenbuterol - 100%, Arubendol - <1%, Mabuterol - <1%, Brombuterol - <1%, Salbutamol - <1%, Ractopamine - <1%, Sensitivity: 0.3 ppb (ng/mL)
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 12 Months |
Product Details
| 96Trace CAT | E4771-100 |
| Supplier CAT | TRC-00228 |
| Analyte / Target | Cimaterol |
| Category | Toxicology & Food Safety ELISA Kits |
| Alternate Name | 3110 |