Chlortetracycline ELISA Kit

Product Overview

A competitive ELISA kit for the quantitative measurement of Chlortetracycline in Tissue, Liver, Eggs, Honey, Urine.

Chlortetracycline is an antibiotic produced by some strains of Streptomyces aureofaciens. It inhibits protein synthesis (elongation) by preventing binding of aminoacyl-tRNA to the 30S subunit. It is effective against Gram-positive and to a lesser degree Gram-negative bacteria than tetracycline. 96Trace Chlortetracycline ELISA Kit is based on Competitive ELISA principle. The micro-plate provided in this kit has been pre-coated with Chlortetracycline. During the reaction, Chlortetracycline in the samples or standard competes with Chlortetracycline coated on the plate for binding to the anti-Chlortetracycline antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of Chlortetracycline. The concentration of Chlortetracycline in the samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Information

Performance Data

Applications

This ELISA kit is used for quantitative measurement of Chlortetracycline in Tissue, Liver, Eggs, Honey, Urine.

Features & Benefits

• Designed for quantitative measurement of Chlortetracycline.
• Assay format: Competitive ELISA.
• Sample type: Tissue, Liver, Eggs, Honey, Urine.
• Detection method: Absorbance (450 nm).
• Detection Range: Tissue, Liver, Eggs - 0.8 ppb, Honey - 4 ppb, Urine -1 ppb, Sensitivity: 0.1 ppb, Cross-reactivity: Chlortetracycline - 100%, Tetracycline -29%, Oxytetracycline -15%, Doxycycline -2.5%.
• Sensitivity: 0.1 ppb, Cross-reactivity: Chlortetracycline - 100%, Tetracycline -29%, Oxytetracycline -15%, Doxycycline -2.5%.
• Kit size: 96 Well.

Storage & Shipping

Product Details