cGMP ELISA Kit

Product Overview

A Competitive ELISA kit for in vitro quantitative determination of cGMP concentrations in serum, plasma and other biological fluids

cGMP ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with cGMP. During the reaction, cGMP in the sample or standard competes with a fixed amount of cGMP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to cGMP. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of cGMP in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Information

Performance Data

Applications

Specificity: This kit recognizes cGMP in samples. No significant cross-reactivity or interference between cAMP and analogues was observed., Detection Range: 0.78-50 pmol/ml, Repeatability: Coefficient of variation is < 10%., Sensitivity: 0.47 pmol/ml

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