TRC-00216 | cGMP ELISA Kit | 96 Well
Product Overview
A competitive ELISA kit for the quantitative measurement of cGMP in Plasma, Serum, Other biological fluids.
cGMP ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with cGMP. During the reaction, cGMP in the sample or standard competes with a fixed amount of cGMP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to cGMP. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of cGMP in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Information
| Assay Format | Competitive ELISA |
| Species Reactivity | All |
| Sample Type | Plasma, Serum, Other biological fluids |
| Detection Method | Absorbance-450 nm |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 0.78-50 pmol/ml, Repeatability: Coefficient of variation is < 10%., Sensitivity: 0.47 pmol/ml |
| Sensitivity | 0.47 pmol/ml |
Applications
This ELISA kit is used for quantitative measurement of cGMP in Plasma, Serum, Other biological fluids.
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 12 Months |
Product Details
| Catalog Number | TRC-00216 |
| Analyte / Target | cGMP |
| Alternate Name | Guanosine 3',5'-cyclic phosphate, Cyclic GMP, 3',5'-cyclic GMP, Guanosine cyclic monophosphate;, Cyclic 3',5'-GMP |