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TRC-00191 | CD14 (Human) ELISA Kit | 96 Well

Product Overview

A rapid sandwich ELISA kit for the quantitative measurement of CD14 in human serum, plasma, cell culture supernatnat and other biological fluids

Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. 96Trace’s CD14 ELISA kit is a sandwich ELISA assay for measuring the quantity of human CD14 in serum, plasma and cell culture and other biological fluid. The entire process can be performed within 90 minutes with fewer incubation and wash step respectively, resulting in less errors and more consistent results.

Assay Information

Assay FormatSandwich ELISA
Species ReactivityHuman
Sample TypeHuman serum, plasma, tissue homogenates and other biological fluids.
Detection MethodAbsorbance (450 nm)
Detection Wavelength450 nm
Kit Size96 Well

Performance Data

Detection Range0.1 - 15 ng/ml
Sensitivity< 0.01 ng/ml
Assay Time90 minutes

Applications

This ELISA kit is used for quantitative measurement of CD14 in human serum, plasma, cell culture supernatant and other biological fluids.

Features & Benefits

  • Easy, convenient and time-saving method to measure the level of CD14 in human serum, plasma, culture supernatants and other biological fluids.
  • The entire assay can be performed within 90 minutes with with fewer incubation and wash step respectively, resulting in less errors and more consistent results.
  • Detection Range: 0.1 - 15 ng/ml
  • Sensitivity: < 0.01 ng/ml
  • Assay Precision: Intra-Assay: CV < 10%; Inter-Assay: CV < 12% (CV (%) = SD/mean X 100)
  • Cross Reactivity: No significant cross-reactivity or interference between this analyte and its analogues was observed.

Storage & Shipping

Storage+4°C
ShippingGel Pack
Shelf Life6 Months

Product Details

96Trace CATK4415-100
Supplier CATTRC-00191
Analyte / TargetCD14
CategoryInfectious Disease ELISA Kits