TRC-00149 | Calcitonin (Human) ELISA Kit | 96 Well
Product Overview
A sandwich ELISA kit for in vitro quantitative measurement of Calcitonin levels in human serum, plasma and other biological fluids.
96Trace’s Human Calcitonin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CEA. This assay employs an antibody specific for human Calcitonin coated on a 96-well plate. Standards and samples are pipetted into the wells and Calcitonin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti- human Calcitonin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Calcitonin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Assay Information
| Assay Format | Sandwich ELISA |
| Species Reactivity | Human |
| Sample Type | Plasma, Serum, Other biological fluids |
| Detection Method | Absorbance (450 nm) |
| Detection Wavelength | 450 nm |
| Kit Size | 96 Well |
Performance Data
| Detection Range | 15.63-1000 pg/mL |
| Sensitivity | 9.38 pg/mL, Detection Range: 15.63-1000 pg/mL |
Applications
3110
Features & Benefits
precision:Coefficient of variation is < 10%., Specificity: No Significant cross-reactivity or interference between Human Calcitonin and analogues was observed., Sensitivity: 9.38 pg/mL, Detection Range: 15.63-1000 pg/mL
Storage & Shipping
| Storage | +4°C |
| Shipping | Gel Pack |
| Shelf Life | 12 Months |
Product Details
| 96Trace CAT | E4743-100 |
| Supplier CAT | TRC-00149 |
| Analyte / Target | Calcitonin |
| Category | Cytokine & Growth Factor ELISA Kits |
| Alternate Name | Thyrocalcitonin |