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TRC-00130 | BAFF (Human) ELISA Kit | 96 Well

Product Overview

A rapid sandwich ELISA kit for the quantitative measurement of BAFF in human serum, plasma, cell culture supernatnat and other biological fluids

B-Cell Activating Factor (BAFF) is a cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. 96Trace’s BAFF ELISA kit is a sandwich ELISA assay for measuring the quantity of human BAFF in serum, plasma and cell culture and other biological fluid. The entire process can be performed within 90 minutes with fewer incubation and wash steps respectively, resulting in less errors and more consistent results.

Assay Information

Assay FormatSandwich ELISA
Species ReactivityHuman
Sample TypeHuman serum, plasma, tissue homogenates and other biological fluids.
Detection MethodAbsorbance (450 nm)
Detection Wavelength450 nm
Kit Size96 Well

Performance Data

Detection Range15 - 1000 pg/ml
Sensitivity< 4.5 pg/ml
Assay Time90 minutes

Applications

This ELISA kit is used for quantitative measurement of BAFF in human serum, plasma, cell culture supernatant and other biological fluids.

Features & Benefits

  • Easy, convenient and time-saving method to measure the level of BAFF in human serum, plasma, culture supernatants and other biological fluids.
  • The entire assay can be performed within 90 minutes with with fewer incubation and wash step respectively, resulting in less errors and more consistent results.
  • Detection Range: 15 - 1000 pg/ml
  • Sensitivity: < 4.5 pg/ml
  • Assay Precision: Intra-Assay: CV < 10%; Inter-Assay: CV < 12% (CV (%) = SD/mean X 100)
  • Cross Reactivity: No significant cross-reactivity or interference between this analyte and its analogues was observed.

Storage & Shipping

Storage+4°C
ShippingGel Pack
Shelf Life6 Months

Product Details

96Trace CATK4423-100
Supplier CATTRC-00130
Analyte / TargetBAFF
CategoryCancer Biomarker ELISA Kits