Anti-Rituximab (Mabthera®) (Human) ELISA Kit

Product Overview

A Sandwich ELISA kit for the quantitative measurement of antibody against Rituximab in human serum or plasma.

Rituximab (Mabthera®) is a genetically engineered chimeric murine/human monoclonal antibody specific to CD20. CD20 is an approximately 35 KDa transmembrane phosphoprotein involved in the activation, proliferation, and differentiation of B-lymphocytes. It is absent in terminally differentiated plasma cells. The Fab domain of rituximab binds to the CD20 antigen on B-lymphocytes and the Fc domain recruits immune effector functions to induce apoptosis in B cells. It is used in treating leukemias and lymphomas, some autoimmune disorders, and organ transplant. However, some patients develop unwanted immunogenicity, which leads to production of anti-drug-antibodies (ADAs) inactivating the therapeutic effects of the treatment and, in rare cases, inducing adverse effects. 96Trace’s BioSimTM anti-Rituximab ELISA kit is designed to quantify/measure the antibody against Rituximab with high specificity and sensitivity in biological matrices.

Assay Information

Performance Data

Applications

This ELISA kit is used for quantitative measurement of antibody against Rituximab in human serum and plasma

Features & Benefits

• Easy, convenient, sensitive and time-saving method to measure the level of antibody against Rituximab in human serum and plasma.
• Detection Range: 62 - 500 ng/ml
• Sensitivity: 30 ng/ml
• Assay Precision: Intra-Assay: CV < 15%; Inter-Assay: CV < 15% (CV (%) = SD/mean X 100)
• Cross Reactivity: Rituximab (Rituxan®, Mabthera®) infusion camouflages/masks the presence of antibody to rituximab (ATR) in serum/plasma samples. Therefore, blood sampling time is critical for detection of ATR. It is convenient to obtain blood sample just before the infusion or at least 2 weeks after the infusion of Rituximab (Rituxan®, Mabthera®)
• Recovery rate: 85 – 115% with normal human serum samples with known concentrations

Storage & Shipping

Product Details